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"Another study has shown EGCG to inhibit LPS induced IkappaBalpha degradation, NF-kappaB nuclear translocation, and NF-kappaB DNA binding activity in bone marrow derived macrophages (BMMs) [XREF_BIBR]."

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"Compare with the group of LPS induced RAW 264.7 macrophages, LPS induced p-IkappaB-alpha and p-p65 protein binding activity was significantly reduced in cultures treated with CG at dose of 100 muM."

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"As shown in XREF_FIG, 8f effectively reduced LPS induced IkappaB-alpha degradation at a concentration of 10 microM."

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"When cells were pre-incubated with TPCK (10 mu m) this LPS mediated degradation of IkappaB-alpha was completely blocked."

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"Further, LPS induced IkappaBalpha degradation was not affected by acute alcohol consumption indicating an IkappaBalpha independent mechanism, as observed earlier in the in vitro acute alcohol studies."

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"C also significantly attenuated the amount of LPS induced IkappaB-alpha degradation (p <.05), albeit not to the same degrees as the inhibitors."

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"First, antecedent exposure of alveolar macrophages to S/R resulted in increased LPS induced IkappaBalpha degradation through activation of upstream signaling, an effect that resulted in increased NF-kappaB translocation and cytokine induced neutrophil chemoattractant gene expression."

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"FA did not attenuate COX-2 expression at a concentration of 100 muM, however, both genistein and FA15 (10 muM each) did (IR = 69 and 76%, respectively), though each were inactive at 2 muM.We next investigated the suppressive effects of FA15 on LPS and IFN-gamma-induced degradation of IkappaB-alpha."

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"Our results demonstrated that LPS caused rapid degradation of IkBalpha, while MSS1 gene knockdown significantly reduced IkBalpha degradation in LPS stimulated BV2 microglia (XREF_FIG)."

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"We found that CIS promotes LPS induced IkappaBalpha degradation and enhances NF-kappaB activity in cholangiocytes."

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"We found that SC extract attenuated LPS induced IkappaB-alpha degradation as well as nuclear translocation of p65 in BV-2 microglia, indicating that SC extract inhibits iNOS and TNF-alpha gene expression in microglia and may be involved in the inhibition of NF-kappaB activation as a possible mechanism."

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"We show here that pretreatment of lipopolysaccharide (Salmonella abortus equi, 2.5 mg/kg)-challenged mice with ANP (5 microg/kg iv, 15 min) rapidly inhibits nuclear factor-kappaB activation via inhibition of phosphorylation and degradation of the IkappaB-alpha protein."

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"In a recent study, Wei et al. [36] revealed that rgcIL-10 (recombinant grass carp IL-10) and rgcTGF-beta1 (recombinant grass carp TGF-beta1) could block LPS induced IkappaBalpha protein degradation in grass carp monocytes and macrophages."

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"In Western blotting, pretreatment with erdosteine inhibited the IkBalpha degradation induced in RAW 264.7 cells by LPS."

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"We confirmed these results in primary WT microglia transfected with PKCdelta specific siRNA to show that PKCdelta knockdown attenuated LPS induced IkappaBalpha degradation (XREF_FIG), which is consistent with our findings using PKCdelta deficient microglia."

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"In contrast, LPS stimulation triggered IkappaBalpha degradation after 1 hr in MMNK-1 cells and this was not impacted by TGFbeta1 cotreatment (XREF_FIG)."

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"CORM-3 did not influence P. intermedia LPS induced degradation of IkappaB-alpha."

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"Interestingly, the exposure of non CF HBG cells to P. aeruginosa LPS plus genistein prevented the P. aeruginosa LPS induced degradation of IkappaBalpha in cells."

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"Thus, poly I induced an increase in nuclear factor kappaB (NF-kappaB) transcriptional activity due to a long-term degradation of inhibitory NF-kappaB (IkappaB) beta while lipopolysaccharide (LPS) induced the degradation of both IkappaBalpha and IkappaBbeta."

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"A6 inhibited the LPS induced OC differentiation, TNF-alpha production, and IkappaBalpha degradation in the control condition, whereas A6 had no effects on them in the uPA knockdown condition."

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"WIN-34B significantly inhibited the LPS induced degradation of IkappaB-alpha at a concentration of 100 mug/ml (XREF_FIG A)."

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"Meanwhile, the most active compounds, 3a (XREF_FIG) and 3c (XREF_FIG), dose-dependently reduced the LPS induced IkappaBalpha degradation, though 3c at 5 muM has no significant effect."

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"As shown in Figure XREF_FIG, neither a MLK3 inhibitor, K252a nor a JNK inhibitor, SP600125 had any effect on LPS induced IkappaB-alpha degradation or NF-kappaB transcriptional activity."

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"XREF_BIBR Addition of antioxidants or overexpression of peroxidases in the same stressed cells blocked IkappaBalpha degradation induced by TNF, PMA and LPS."

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"Heat shock, 1 h before treatment with LPS, completely inhibited LPS mediated degradation of I-kappaBalpha."

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"To rule out the possibility that this decrease was due to the loss of PP4 's known negative regulation of LPS induced NFkappaB activation XREF_BIBR, we examined LPS induced IkappaBalpha degradation in WT and CD23 cre PP4 F/F B cells but found no abnormalities in the mutant cells (XREF_SUPPLEMENTARY)."

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"LPS challenge caused IkappaB-alpha degradation in both WT endothelial cells (XREF_SUPPLEMENTARY) and -transfected cav-1 -/- endothelial cells transfected with WT-Cav-1 (XREF_FIG)."

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"Using Western blot analysis, TPCK was found to actually inhibit LPS mediated degradation of IkappaB-alpha."

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"UA also inhibited LPS mediated I-kappaBalpha degradation."

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"We also explored whether blue pigments inhibited the LPS stimulated degradation of IkappaB-alpha in RAW 264.7 cells by Western blotting with anti-IkappaB-alpha antibody."

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"Stimulation with hMRP8 or LPS caused IkappaBalpha degradation as represented by substantially reduced total IkappaBalpha protein and activated NF-kappaB p65 in naive cells; however, pre-stimulation with hMrp8 failed to prevent hMrp8- or LPS induced IkappaBalpha degradation (XREF_FIG) and NF-kappaB p65 phosphorylation (XREF_FIG)."

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"In lean subjects, LPS induced IkBalpha activation was not significant compared to unstimulated control myotubes, whereas PMI 5011 mediated inhibition of LPS induced activation of IkBalpha was significant."

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"In addition, we found that AMPK negatively regulated LPS induced IkappaB-alpha degradation and positively regulated Akt activation, accompanied by inhibition of GSK3-beta and activation of CREB."

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"The specificity of this effect was again indicated by the lack of IkappaBalpha degradation in cells treated with the LPS contamination control (XREF_FIG, lane 3)."

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"These data indicated that gene silencing of C5aR with siRNA protected from LPS induced NF-kappaB activation and nuclear translocation.To clarify whether the C5aR effect on NF-kappaB nuclear translocation is due to the regulation of the NF-kappaB inhibitor IkappaBalpha, we transfected endothelial cells with C5aR-siRNA and then added LPS for different times to follow LPS induced IkappaBalpha degradation."

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"DEX prevented the LPS induced IkappaBalpha degradation, which was also reversed by exogenous PAF."

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"Moreover, LPS treatment caused prominent IkappaB-alpha degradation as compared to the untreated control cells; nevertheless, PEF pretreatment notably recovered the protein level of IkappaB-alpha (XREF_FIG)."

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"As expected 3, robust LPS induced I-kappaBalpha degradation and activation of IKK and ERK were observed in naive macrophages but strongly blunted in TNF tolerized macrophages (XREF_FIG)."

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"In contrast to LPS tolerized cells, in TNF tolerized macrophages the initial phase of LPS induced I-kappaBalpha degradation was nearly intact (XREF_FIG, lanes 9-12 vs. 5-8)."

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"1,8-Cineole and rosiglitazone blocked the LPS induced IkappaBalpha degradation and NF-kappaB p65 nucleus translocation, which could be reversed by the pretreatment of GW9662 or silence of PPAR-gamma gene."

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"LPS induced IkappaBalpha degradation in control cells as early as 20 min but in co-treated cultures the degradation of IkappaBalpha was not evident."

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"Furthermore, RP-L blocked LPS induced degradation of IkappaB-alpha and nuclear accumulation of p65."

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"This crude drug decreased LPS induced IkappaBalpha degradation in a concentration dependent manner without affecting LPS induced IkappaB phosphorylation."

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"Furthermore, the compound inhibited LPS mediated nuclear translocation of NF-kappaB p65 and DNA binding activity of NF and kappaB complex, in parallel, but did not influence LPS mediated IkappaBalpha degradation."

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"The experimental evidence obtained in RAW 264.7 macrophages indicating that IDR-1002 inhibits LPS induced COX-2 expression, activates CREB phosphorylation at Ser133, and inhibits LPS induced IkappaBalpha degradation was confirmed in PBMC."

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"Consistent with these findings, FACScan analysis further revealed that hMrp8 pre-stimulation significantly downregulated both hMrp8- and LPS induced p38 phosphorylation, but failed to attenuate hMrp8- or LPS induced IkappaBalpha degradation and NF-kappaB p65 activation."

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"Both E. coli LPS and P. gingivalis LPS induced IkappaBalpha degradation in human gingival fibroblasts."

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"Furthermore, LPS induced IkappaBalpha degradation, DNA binding, and NF-kappaB-dependent gene transcription activation were abrogated by short-term heat."

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"Since TIRAP is reportedly a proximal signaling mediator that functions upstream of IkappaB degradation, the ability of TIRAP-D/N to inhibit LPS induced IkappaBalpha degradation in EC was assayed."

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"As expected, an almost complete loss of phospho- IkappaBalpha as well as IkappaBalpha was identified in macrophages treated with LPS alone."

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"These data show that LPS treatment activated NF-kappaB by promoting the degradation of IkappaBalpha and the nuclear localization of NF-kappaBp65, which induced MUC5AC overproduction."

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"Furthermore, treatment with the PKC inhibitor abrogated LPS induced IkappaBalpha degradation and NF-kappaB activation, while treatment with the PTP inhibitor enhanced LPS induced IkappaBalpha degradation and NF-kappaB activation in p-AMs, suggesting that PKC and PTP are regulators of LPS induced IkappaBalpha degradation and NF-kappaB activation."

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"LPS induced IkappaBalpha degradation greatly varied over time, whereas that induced by TGF-alpha gradually increased to reach maximal levels after 1 h of stimulation after which IkappaBalpha expression increased."

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"LPS treatment of B cells triggers the rapid degradation of IkappaBalpha followed by the nuclear translocation of cytoplasmic p50-RelA and p50-c-Rel heterodimers, both in 70Z/3 and primary splenic B cells."

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"Effect of quercetin on P. intermedia LPS induced IkappaB-alpha degradation."

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"Thus, poly I induced an increase in nuclear factor kappaB (NF-kappaB) transcriptional activity due to a long-term degradation of inhibitory NF-kappaB (IkappaB) beta while lipopolysaccharide (LPS) induced the degradation of both IkappaBalpha and IkappaBbeta."

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"Figure 3A and B show that LPS dramatically stimulated IkappaB-alpha degradation by 30 min after the start of treatment and that this degradation was clearly inhibited by the bis -eugenol treatment."

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"In addition, it was reported that PHOX inhibitors prevented LPS and IFNgamma induced degradation of IkappaBalpha, and thus, inhibited the activation of NF-kappaB."

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"In addition, the ability of EGL to block the LPS stimulated degradation of IkappaB-alpha was investigated by western blotting."

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"Besides, CDM did not affect the kinetic of IkappaBalpha degradation induced by LPS."

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"In addition, in vitro LPS stimulation (50 microg ml (-1)) significantly induced NF-kappaB activation and cytoplasmic IkappaBalpha degradation in Mphi, enhanced TNF-alpha mRNA levels and increased Mphi TNF-alpha and MAL."

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"Degradation of I-kappaBalpha by LPS was significantly abolished in cells pretreated with arctigenin at the concentration of 1 muM."

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"Furthermore, CHL partially restored LPS decreased IkappaBalpha, an inhibitory protein against NF-kappaB activation, in the cytosolic extract from the LPS treated cells determined by immunoblot analysis."

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"Next, we investigated whether genistein inhibits LPS induced degradation of IkappaB-alpha in RAW 264.7 macrophages by Western blotting with anti-IkappaB-alpha antibody."

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"As expected, IkappaBalpha degradation was observed at 15 and 30 minutes in cells treated with LPS (XREF_FIG, lanes 2 and 3); however there was no significant effect of Misoprostol on the rate or the level of IkappaBalpha degradation (XREF_FIG; compare lanes 2, 3 and 6, 7)."

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"In the present study, using murine macrophages, we clearly demonstrate that erucin inhibits LPS induced degradation of IkappaB-alpha, translocation of p65 from the cytosol to nucleus and NFkappaB DNA biding activity and NFkappaB reporter activity (XREF_FIG)."

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"In the present study, we observed that LPS challenge induced the degradation of IkappaB-alpha and activation of NF-kappaB in vitro, and the activation of NF-kappaB signaling was further elevated by ERRalpha knockdown, suggesting that activation of NF-kappaB signaling plays an important role in the deteriorative effects of ERRalpha knockdown on ALI."

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"The extract also inhibited LPS induced IkappaBalpha degradation and MAPK activation as well as LPS and IFN-gamma-induced STAT1 activation, effects observed at a higher concentration than that required to suppress iNOS."

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"LPS induced IkappaBalpha degradation and increased levels of NF-kappaB p65 subunit in the nucleus."

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"In our study, LPS induced degradation of IkappaB-alpha and NF-kappaBp65 nuclear localization in BMDCs from Wnt5aCAGDelta and Delta and Ror2 MxDelta and Delta mice, as well as BMDCs from control mice."

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"Exploration of the mechanisms by which GSK-3beta positively regulates inflammatory response showed that LPS induced IkappaB-alpha degradation, NF-kappaBp65 nuclear translocation, and p65 DNA binding activity were not affected by inhibition of GSK-3beta activity."

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"In addition, it has been reported that PHOX inhibitors prevent LPS and IFNgamma induced degradation of IkappaBalpha, and thus inhibit activation of NF-kappaB [XREF_BIBR]."

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"Earlier it was shown that in macrophage cell lines, constitutively expressed AMPK reduced LPS stimulated IkappaBalpha (inhibitor of NF-kappaB) degradation and p65 binding to the IL-6 promoter."

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"LPS also stimulated IkappaBalpha degradation in rBDECs after 1 hr (data not shown)."

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"CAPE did not interfere with IkappaB-alpha degradation induced by P. intermedia LPS."

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"Furthermore, E5531 was able to inhibit LPS stimulated IkBalpha degradation at 0.1 mug/ml (XREF_FIG d)."

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"The incubation of mouse splenocytes with alpha-cubebenoate prior to LPS stimulation markedly blocked LPS induced IkappaB-alpha degradation (XREF_FIG E)."

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"As shown in XREF_FIG, Shp2 deletion had no effect on LPS stimulation of IkappaBalpha degradation (hence NF-kappaB activaton) in hepatocytes."

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"Here, we report that knockdown of NEMO by RNA interference in Jurkat E6.1 cells enhanced TCR induced NF-kappaB report gene activity and IL-2 production by promotion of IkappaBalpha degradation and p65 nuclear translocation, whereas inhibited TNF-alpha and LPS induced IkappaBalpha degradation without influencing the phosphorylation of MAPKs."

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"LPS induced IL-8 production was determined by enzyme linked immunosorbent assay (ELISA), and LPS induced IkappaB-alpha degradation was followed by Western blot analysis."

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"In the meanwhile, LPS treatment did not induce degradation of IkappaBalpha protein in p38alpha -/- astrocytes (XREF_FIG)."

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"Consistent with this finding, BE inhibited LPS induced IkappaBalpha degradation, NF-kappaB nuclear translocation, and also Akt, c-Jun NH2-terminal kinase (JNK) phosphorylation."

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"PYE inhibited LPS induced TNF-alpha and IL-6 in macrophages at the transcriptional level and also suppressed LPS induced IkappaBalpha degradation and MAPK activation in vitro."

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"Bacterial LPS induced IkappaBalpha degradation in human gingival fibroblasts was investigated by western blot."

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"Although LPS treatment also induced the degradation of IkappaBalpha, the kinetic pattern was different from that of PCSC."

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"When added at the same concentration, clasto -lactacystin beta-lactone also inhibited LPS induced degradation of IkappaB-alpha in the CHO-CD14 cells (data not shown)."

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"However, we observed no change in the kinetics of LPS induced IkappaBalpha degradation in Ptpn22 -/- BMM (XREF_SUPPLEMENTARY)."

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"In addition, both EGCG and GCG inhibited LPS induced degradation of IkappaBalpha with concomitant inhibition of nuclear protein binding to NF-kappaB site and synthesis of IRF-1."

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"We also confirmed that A6 attenuated the LPS induced IkappaBalpha degradation."

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"DBZ suppressed the LPS induced degradation of IkappaBalpha, thereby decreasing the translocation of p65 to nucleus."

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"However, LPS and LSECtin mAb combined treatment induces IkappaBalpha degradation that last up to 2h."

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"PPARgamma ligand delayed LPS induced IkappaBalpha degradation."

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"To address the possibility that the impaired nuclear translocation of p65 was due to inhibition of degradation of IkappaB-alpha, we examined the effect of ATL on IkappaB-alpha degradation induced by LPS."

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"Similar to these previous findings, we show here that dDAVP inhibited both the LPS induced degradation of IkappaB-alpha and its stimulation of phosphorylated p65 and RelA, as well as LPS mediated NF-kappaB activation and cytokine secretion in mpkCCD cl4 cells."

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"Degradation of IkappaB-alpha protein by LPS was almost completely abolished in RAW 264.7 cells treated with these compounds at 10 or 30 muM concentrations."

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"Within 15 min, LPS alone rapidly induced degradation of IkappaBalpha, levels of which returned to normal within 30-5 min."

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"This occurred at the transcriptional level, since the gingerol compounds decreased LPS induced IkappaB-alpha degradation, prevented nuclear translocation of NF-kappaB p65 and reduced NF-kappaB activity in a concentration dependent manner."

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"Thus, shRNA against TRPV2 inhibited the LPS induced mRNA for tumor necrosis factor-alpha (TNF-alpha) and interleukin (IL) -6 and induced inhibitor of nuclear factor-kappaB (NF-kappaB) alpha (IkBalpha) degradation."

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"Interestingly, NFkappaB activation of B lymphocytes by LPS occurs rapidly and is likely initiated by LPS induced IkappaBalpha degradation, which occurs in 1-1.5 hr."

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"In addition, the 2h P. amarus pretreatment significantly blocked the degradation of LPS induced IkappaBalpha in a concentration dependent manner (Additionalfile2 : Figure S2)."

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"However, LPS and IFN-gamma-induced degradation of I-kappaBalpha was attenuated in the presence of C-1."

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"Although the initial IkappaB-alpha degradation induced by LPS was minimally affected by beta-agonists, the latter induced a marked rebound of the cytosolic IkappaB-alpha levels at later time points (> 1 h), accompanied by an increased IkappaB-alpha cytoplasmic half-life."

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"THC also significantly suppressed LPS induced release of pro inflammatory cytokines and degradation of IkappaB-alpha."

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"Moreover, CSM and LPS (as a control) degradated IkappaB-alpha and preincubation of MDMs with NAC suppressed the degradation of IkappaB-alpha induced by CSM."

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"AMWAP prevents LPS triggered degradation of IRAK-1 and IkappaBalpha."

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"Zn250 also attenuated the LPS induced IkB-alpha degradation."

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"Besides, LPS induced IkappaBalpha degradation was little affected either (XREF_FIG), indicating that NCTD advanced NF-kappaB activation was not through IkappaBalpha degradation."

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"Bacterial LPS induces persistent NF-kappaB activation by degrading IkappaB-alpha as well as IkappaB-beta in responsive cells."

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"15-A 3t -IsoP blocked the LPS stimulated IkappaBalpha degradation both at 15 min as well as at 30 min (XREF_FIG)."

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"PL has been shown to inhibit LPS induced IkappaBalpha degradation and activation of NF-kappaB and ERK in liver [XREF_BIBR]."

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"We next determined whether NFD-37 could affect LPS induced IkappaBalpha degradation, using Western immunoblot analysis with cytoplasmic extracts of murine macrophages RAW 264.7."

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"Conflicting data exists on whether parthenolide inhibits LPS induced IkappaBalpha degradation, while the effect on IkappaBbeta degradation has never been reported."

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"However, curcumin abolished the LPS induced IkappaBalpha degradation."

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"CSE or LPS (the positive control) increased IkappaB-alpha phosphorylation (XREF_FIG) and exposure to CSE or LPS resulted in the degradation of IkappaB-alpha (XREF_FIG)."

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"BHBA was found to inhibit the LPS induced degradation of IkappaB-alpha."

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"As shown in Fig. 2, 1-BP suppressed the IkappaBalpha degradation induced by IL-1 or LPS in astrocytes without influence on beta-actin."

eidos
"Western blots revealed that IkappaB-alpha ( NF-kappaB inhibitor alpha ) degraded 1 h after LPS administration and began to restore after 3 h , while TP pretreatment inhibited LPS induced IkappaB-alpha degradation 1 h after LPS injection ( Fig. 4H and L ) ."

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"In addition, THC significantly attenuated LPS induced degradation of IkappaB-alpha in LPS induced rat mesangial cells."

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"C also reduced LPS induced IkappaB-alpha degradation and NF-kappaB translocation."

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"Recently, BAI has been shown to inhibit inflammation through inhibition of COX-2 gene expression [XREF_BIBR] and to suppress LPS induced degradation of IkappaBalpha and activation of NF-kappaB [XREF_BIBR]."

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"Pretreatment of rats with pyrrolidine dithiocarbamate (50, 100, and 200 mg/kg, i.p.), an inhibitor of NF-kappaB activation, prevented LPS induced I-kappaBalpha degradation and the resultant NF-kappaB activation and inhibited, in a dose related manner, the LPS induced CINC and ICAM-1 mRNA and protein expression."

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"As shown in Figure 4A XREF_FIG, treatment with LPS induced IkappaBalpha degradation, which was significantly blocked by SFEIO."

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"It has been shown previously that LPS treatment causes degradation of IkappaBalpha protein within 40 minutes, followed by induction of IkappaBalpha mRNA that results in rapid recovery of the IkappaBalpha protein by 3 hours."

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"We also confirmed that uPA, plasmin, TFLLRN-NH 2, and AICAR attenuated the LPS induced IkappaBalpha degradation."

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"This indicates LPS induced IkappaBalpha degradation (XREF_FIG)."

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"However, these compounds did not prevent the DNA binding activity of NF-kappaB assessed by electrophoretic mobility shift assay as well as the induced degradation of IkappaB-alpha protein by LPS or TNF-alpha."

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"In order to investigate whether LPS is able to activate NF-kappaB via intracellular TLR4, we examined degradation of IkappaB-alpha in NB-1 cells treated with LPS (100 ng/ml) for various time."

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"Treatment with flavonoids blocked LPS induced IkBalpha degradation."

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"In the present study, we found LPS challenge induced the degradation of IkappaB-alpha and activation of NF-kappaB in vivo and in vitro, and the activation of NF-kappaB signaling was inhibited by DPQ treatment, suggesting that inhibition of NF-kappaB signaling plays a role in the protective effects of DPQ on ALI."

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"We also used western blotting to determine whether SF would block LPS stimulated degradation of IkappaB-alpha."

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"The present study showed that both LPS administration and the fcHFHS diet decreased hypothalamic NFKBIA protein and increased Nfkbia mRNA."

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"NCX 2121 did not display inhibitory action towards IkappaB-alpha degradation triggered by LPS."

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No evidence text available

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"In addition we have shown that the compound inhibited the LPS and IFN-gamma mediated degradation of the IkappaB-alpha protein and subsequent activation of the NF-kappaB transcription factor."

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"Misoprostol does not affect LPS induced IkappaBalpha Degradation and NFkappaB Nuclear Translocation."

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"LPS induced IkappaBalpha degradation (~ 0.4 fold) was recovered in CMCE (1 and 10 mug/mL) pre-treated THP-1 cells in a dose dependent manner (~ 1.8 and 2.3 fold, respectively)."

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"However, as the precise timing and mechanism of LPS and nigericin activation of the Nalp3 (NLRP3) inflammasome is poorly understood, it is also possible that the lack of activated caspase-1 was due to altered TLR signaling in heat shock conditions, as reflected by heat shock 's inhibitory effect on LPS mediated IkappaB-alpha breakdown (data not shown)."

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"To confirm degradation of IkappaBalpha by the proteasome, we used the proteasome inhibitor MG-132, which reversed IkappaBalpha degradation in all samples, demonstrating that LPS induced IkappaBalpha degradation was dependent on the proteasome (XREF_FIG)."

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"Previous studies have shown [XREF_BIBR, XREF_BIBR], cilostazol inhibits proinflammatory factor (including remnant lipoprotein particle, TNFalpha and LPS)-stimulated increases in IkappaBalpha degradation and NF-kappaB p65 activity, and thus, suppresses the productions of inflammatory cytokines."

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"Aspirin pretreatment also abrogated LPS induced degradation of IkappaBalpha in p-AMs."

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"Parthenolide inhibits LPS induced IkappaBalpha degradation and NF-kappaB activation in RAW 264.7, peripheral blood mononuclear, and vascular smooth muscle cells."

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"Similarly, CyP prevented LPS induced IkappaBalpha degradation and subsequent induction, both indicative of NF-kappaB activation (XREF_FIG)."

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"Taken together, these results suggest that BMS-345541 or wortmannin block LPS induced IkappaBalpha degradation."

eidos
"S9 Fig shows that LPS induced a significant reduction in IkappaBalpha that last up to 1h ."

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"We also used the LPS induced degradation of IkappaBalpha as a measure of signaling function, as this event does not appear to require tyrosine kinase activity and therefore should be intact."

eidos
"LPS stimulation resulted in significantly increased level of NF-kB p65 phosphorylation and decreased level of IkBa expression in normal PMVECs ."

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"However, these effects were significantly reversed by OEA (40muM) at 60min of LPS stimulation, which indicated that OEA inhibited LPS induced IkappaBalpha degradation in THP-1 cells."

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"Consistent with our previous data, we found that nanoparticle treatment functioned to attenuate LPS induced I k B-alpha degradation and NF-kB and p65 trans-location from the cytoplasm to the nucleus (XREF_FIG)."

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"Emodin inhibited the LPS induced degeneration of I kappaB-alpha by corneal fibroblasts in a dose dependent manner (P < 0.05)."

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"LPS caused a significant decrease in both IRAK1 and IkappaBalpha which was not prevented by IV rimonabant."

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"Liao et al XREF_BIBR demonstrated that RvD1 can suppress LPS induced IkappaBalpha degradation and NF-kappaB p65 nuclear translocation, a process which is central to the TNF-alpha induced signalling XREF_BIBR."

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"Thiacremonone also inhibited LPS induced degradation of IkappaB-alpha (increase phosphorylation) in RAW 264.7 cells."

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"In the present study, an analysis of the whole cell extracts showed that the P.g. LPS induced IkappaBalpha degradation in fibroblasts was paralleled by a P.g. LPS induced increase in CatB in the late culture period; furthermore, the P.g. LPS induced IkappaBalpha degradation was prevented by CA-074Me pretreatment (XREF_FIG)."

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"In addition, we found that LPS mediated degradation of IkappaBalpha was markedly less pronounced in macrophages that were pre-treated with FtL or apoferritin (XREF_FIG)."

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"LPS induced IkappaBalpha Degradation Is Intact in XLA PBMCs."

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"As prior BCA (20 muM) treatment effectively prevented LPS induced IkappaBalpha degradation, it is suggested that BCA prevented LPS induced IkappaBalpha degradation and NF-kappaB activation, and decreased iNOS protein expression and NO production.It has been reported that IkappaBalpha protein phosphokinase can phosphorylate IkappaBalpha and thereby target it for degradation through the ubiquitin-proteasome pathway [35]."

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"Although LPS diminished AMPK phosphorylation, metformin or AICAR was able to partially decrease the effects of LPS and toll like receptor 4 (TLR4) engagement on downstream signaling events, particularly LPS induced IkappaBalpha degradation."

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"Au (III) and Pd (II) enhanced LPS induced IkappaBalpha degradation, but Hg (II) and Ni (II) suppressed it."

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"NFD-37 (10 muM) inhibited the LPS induced IkappaBalpha degradation in a time dependent manner."

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"While GG6 reduced LPS induced IkappaB-alpha degradation and showed a trend towards reduced interleukin-1beta release, GG9 prevented the increase in proinflammatory CD80 + macrophage subset, downregulation of the anti-inflammatory CD206 + / CD163 + subset, increase in p38 phosphorylation, and increase in cell bound and secreted interleukin-1beta stimulated by LPS, at least in part through signalling pathways not involving Toll like receptor 4 and nuclear factor-kappaB."

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"LPS induced IkappaBalpha degradation at 30-60 min after treatment, but did not induce IkappaBbeta degradation up to 120 min."

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"To do this, we analyzed MyD88, TRAF6, IKKalpha, IKKbeta, and phospho-IKKalpha and beta (p-IKKalpha and beta), which we found were not affected by infection (XREF_SUPPLEMENTARY), and LPS induced IkappaBalpha degradation (XREF_FIG)."

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"(3) LXA (4) restrained LPS induced IkappaBalpha degradation, NF-kappaB translocation, and NF-kappaB transcriptional activity."

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"It has been reported that LPS challenge (2 mug/ml) can be used to induce the degradation of I k B-alpha and nuclear localization of NF-kB and p65 in cultured RAW264.7 macrophages [XREF_BIBR, XREF_BIBR]."

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"As shown in Figure XREF_FIG, apo-9 '-fucoxanthinone was shown to significantly suppress the LPS induced IkappaB-alpha degradation."

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"Results indicated that LPS significantly stimulated the degradation of IkappaB-alpha, which was effectively prevented by osthole pretreatment in a dose dependent manner (XREF_FIG)."

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"However, pretreatment with 300mug/ml PS+CR significantly decreased the phosphorylation of I-kappaBalpha and blocked the LPS induced degradation of I-kappaBalpha, suggesting that PS+CR may regulate phosphorylation of I-kappaBalpha and I-kappaBalpha expression."

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"Effects of Comp on the Phosphorylation of MAPK and IkappaBalpha, and Degradation of IkappaBalpha by LPS Stimulated BMDCs."

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"Both BAY 11-7085 and parthenolide inhibited LPS induced IkappaBalpha and IkappaBbeta degradation in a dose dependent manner (XREF_FIG)."

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"As shown in Figure XREF_FIG A LPS plus IFNgamma significantly induced the degradation of IkappaBalpha in cytosol, whereas pretreatment of RSV resulted in prevention of the degradation of IkappaBalpha."

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"As shown in Figure XREF_FIG A, LPS induced IkappaB-alpha degradation at 15min after the stimulation."

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"CpdA delayed LPS induced IkappaBalpha reduction reaching its minimum at 120min post-stimulation (XREF_FIG)."

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"Alcohol did not prevent LPS induced IkappaBalpha degradation but decreased the levels of phospho specific IkappaBalpha (Ser32)."

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"LPS and silica both induce degradation of IkappaB-alpha, and MG132 prevents LPS induced, but not silica induced, IkappaB degradation and NF-kappaB activation [63]."

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"LPS induced IkappaBalpha degradation results in NFkappaB translocation, DNA binding, and transactivation of target genes such as TNF-alpha."

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"LPS induced IkappaBalpha degradation was enhanced in cells expressing WT Fcalpha and muR but not mutated Fcalpha and muR (TM-mt) (XREF_FIG)."

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"In contrast substantial I-kappaBalpha degradation was observed in control experiments where the cells were treated with LPS (XREF_SUPPLEMENTARY)."

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"As shown in XREF_FIG, compound 20 dose-dependently reversed LPS induced IkappaBalpha degradation."

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"This is consistent with the observations that 6-MP did not inhibit IkappaB-alpha degradation induced by LPS."

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"To determine whether luteolin interferes with LPS induced IkappaBalpha degradation, the level of IkappaBalpha protein was examined by Western blotting."

reach
"The immunocomplex kinase assay indicated that IkappaB kinase activity stimulated by LPS was inhibited by ceramide, which concomitantly reduced the IkappaBalpha degradation caused by LPS within 1-6 h."

reach
"Thus, we performed Western blotting to determine if TA affects TNF-alpha- or LPS stimulated IkappaB-alpha degradation in different cells."

reach
"In addition, we found that octyl caffeate did not significantly affect IkappaBalpha degradation stimulated by LPS and IFN-gamma in RASMCs."

reach
"CGRP prevented LPS induced IkappaBalpha degradation and NF-kappaB binding to promoters of CXCL1, CXCL8 and CCL2."

reach
"We next examined the effect of CMCE on LPS induced IkappaBalpha degradation in THP-1 cells."

reach
"Immunoblotting showed that carabrol suppressed LPS induced degradation of I-kappaBalpha and decreased nuclear translocation of p65."

reach
"The results showed that LPS stimulation caused degradation of IkappaB-alpha compared with negative control cells (P < 0.05), whereas PF-GPD blocked LPS induced degradation of IkappaB-alpha (P < 0.01), and inhibited NF-kappaB p65 translocation from the cytoplasm to the nucleus, thus interrupting the transcription and translation of inflammatory genes."

reach
"Two hour pretreatment with ZER prevented the degradation of LPS stimulated IkappaBalpha in a concentration dependent manner."

reach
"Additionally, TTN decreased LPS induced IkappaB-alpha degradation (XREF_FIG)."

eidos
"As shown in Figures 6 ( c ) and 6 ( d ) , LPS enhanced the IkappaB-alpha phosphorylation , decreased the levels of IkappaB-alpha , and induced the phosphorylation of p65 ."

reach
"Compounds 1 and 2 suppressed the expression of iNOS and COX-2 and the activity of NF-kappaB through the inhibition of LPS induced I-kappaB-alpha degradation in microglia."

reach
"The degradation of IkappaBalpha was analyzed by western blot, and we observed that the treatment of macrophages with compound 1 prevented the IkappaBalpha degradation induced by LPS stimulation for 15 and 30 min (XREF_FIG)."

reach
"The results showed that treatment of cells with both LPS and dibutyryl cAMP, an analog of cAMP, activated PKA; however, only LPS treatment caused the degradation of IkappaB-alpha and IkappaB-beta."

reach
"LPS treatment of control MDMs reduced IkappaBalpha with a concomitant increase in p-IkappaBalpha (p-IkappaBalpha and IkappaBalpha ratio increased from 0.17 to 0.9)."

reach
"Degradation of I-kappaBalpha protein by LPS was almost completely abolished in the livers of rats pretreated with ACF at the dose of 10 mg/kg."

reach
"We show here that MV and LPS each induced the degradation of IkappaB-alpha, MV enhanced LPS induced IkappaB-alpha degradation."

reach
"To examine the IkappaBalpha degradation, we performed immunocytochemistry on RAW 264.7 macrophages treated with LPS alone or in combination with TDZ for indicated time points."

reach
"As shown in XREF_FIG, LPS stimulation induced the degradation of IkappaB-alpha and activation of NF-kappaB in RAW264.7 cells."

reach
"These results indicate that LPS induces IkappaBalpha degradation by acting at an upstream step to NF-kappaB activation."
Lipopolysaccharide bound to TLR4 inhibits NFKBIA. 1 / 1
| 1

reach
"LPS binding to TLR4 induces IkappaB-alpha degradation, which leads to NF-kappaB activation."
Modified lipopolysaccharide inhibits NFKBIA. 1 / 1
| 1

reach
"We tested the effect of crocin on nuclear factor-kappa B (NF-kappaB) signaling and observed that cells pre-treated with 500muM of crocin exhibited lower levels of LPS induced p-IkappaBalpha, p-IkappaB kinase (IKK) alpha/beta, and p65 expression than those of untreated cells."
SB 203580 affects MAPK14
4 54 | 2 44 5 13
4 54 | 2 44 5 13

tas
No evidence text available

medscan
"The inhibition of the glucose transporter GLUT1 by phloretin notably reduces TXNIP RNA level and the inhibition of the p38 MAP kinase activity by SB203580 reverses the effects of TXNIP on reactive oxygen species-TRX activity."

medscan
"Selective inhibition of p38mitogen-activated protein kinase activity by SB203580 abrogated the IFN-gamma response to adiponectin, whereas extracellular signal-regulated kinase 1/2 inhibition by PD98059 did not affect the response."

tas
No evidence text available

medscan
"Inhibition of p38 mitogen-activated protein kinase by SB203580 counteracted the influence of IL-1beta on Fhl2 and Tubb expression, indicating partial involvement of this signaling pathway."

trips
"This p38 MAP kinase, like p38 alpha, is inhibited by the pyridinyl imidazole drug SB203580."

tas
No evidence text available

tas
No evidence text available

medscan
"Activation of the ERK or p38 mitogen-activated protein kinase pathways with PMA or anisomycin decreased dimerization, an effect that was largely prevented when PMA treatment was combined with U0126, which inhibits the ERK mitogen-activated protein kinase pathway, or anisomycin treatment was combined with SB203580, which inhibits p38 mitogen-activated protein kinase."

medscan
"The inhibition of the p38-kinase by SB203580 or infection with a dominant negative p38 kinase adenovirus significantly increased basal number of endothelial progenitor cells (136.7+/-6.3% and 142.9+/-18% versus control, respectively)."

signor
"Pretreatment of hela cells with sb 203580, a pyridinyl imidazole compound that specifically inhibits p38 mitogen-activated protein kinase (mapk). It has previously been established that sb 203580 acts primarily to block the catalytic activity of p38 mapk. However, it has been suggested that in cells, the compounds could also inhibit p38 mapk activation by virtue of their ability to bind to the inactive enzyme."

reach
"SB203580 is a competitive inhibitor of p38alpha and p38beta by blocking ATP binding to the kinase, while BIRB796 is an allosteric inhibitor of p38alpha, p38beta, and p38gamma."

tas
No evidence text available

reach
"Conversely, the inhibition of MAPK14 by SB203580 and the down-regulation of MAP2K3 and MAP2K6 (kinases upstream of MAPK14) by specific siRNA did not alter the activation of MAPK3/1."

tas
No evidence text available

signor
"Pretreatment of hela cells with sb 203580, a pyridinyl imidazole compound that specifically inhibits p38 mitogen-activated protein kinase (mapk). It has previously been established that sb 203580 acts primarily to block the catalytic activity of p38 mapk. However, it has been suggested that in cells, the compounds could also inhibit p38 mapk activation by virtue of their ability to bind to the inactive enzyme."

medscan
"SB203580 [4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl) 1 H-imidazole; Calbiochem] directly inhibits the activation of p38 mitogen-activated protein kinase, and SP600125 (1,9-Pyrazoloanthrone; Calbiochem) preferentially inhibits anti-c-Jun N-terminal kinase 1/2."

reach
"PD 98059 prevents the activation of MAP kinase kinase-1 [12], the enzyme that activates the MAP kinase family members MAPK1 and ERK1 and MAPK2 and ERK2 in the ` classical ' MAP kinase cascade, while SB 203580 inhibits the MAP kinase family members SAPK2a and p38 and SAPK2b and p38beta2 [13] which are components of a distinct protein kinase cascade (reviewed in [14])."

tas
No evidence text available
| DOI

medscan
"In addition, (-)-epigallocatechin gallate induced phosphorylation of p38 mitogen-activated protein kinase , a stress-inducible kinase believed to negatively regulate tumorigenesis, and the inhibition of p38 mitogen-activated protein kinase by SB203580, a specific p38 mitogen-activated protein kinase inhibitor, or the gene silencing using p38 mitogen-activated protein kinase-small interfering RNA suppressed the internalization and subsequent degradation of EGFR induced by (-)-epigallocatechin gallate."

tas
No evidence text available

medscan
"Of note, the inhibition of ERK or p38 mitogen-activated protein kinase by U0126 or SB203580, respectively, decreased the apoptosis induced by cisplatin; however, enhanced apoptotic effects were observed with the use of ERK/p38 mitogen-activated protein kinase inhibitor in combination with butein."

medscan
"Following pretreatment with mitogen-activated/extracellular signal-regulated kinase kinase (MEK) inhibitor U0126, extracellular signal-regulated kinase (ERK) inhibitor PD098059, and P38 mitogen-activated protein kinase (P38MAPK) inhibitor SB203580 for 30 min, chondrocytes were treated with OPG, and Cell Counting kit-8 was performed."

tas
No evidence text available

tas
No evidence text available

tas
No evidence text available

tas
No evidence text available

tas
No evidence text available

medscan
"Progesterone could repress the activation of iNOS through P38mitogen activated protein kinase pathway within polymorphonuclear leukocytes (P<0.05), which could be blocked by SB203580 (P<0.01), but not by actinomycin D (P>0.05)."

medscan
"We investigated the role of p38 MAP kinase in activating NADPH oxidase stimulated by the bacteria-derived peptide fMLP. fMLP-stimulated superoxide production was completely abolished by SB203580, a p38 MAP kinase inhibitor, whereas anisomycin, a p38 MAP kinase activator, did not induce superoxide production, indicating that p38 MAP kinase was essential, but not sufficient, for NADPH oxidase activation. 1999999={EffectorType="agonist" Target=1432}}"

tas
No evidence text available

tas
No evidence text available

tas
No evidence text available

tas
No evidence text available

tas
No evidence text available

tas
No evidence text available

medscan
"Cells were transfected with 100 nM p38 mitogen-activated protein kinase small interfering RNA or 2 μg mitogen-activated protein kinase kinase 3 expression vector (a specific kinase that directly activates p38 mitogen-activated protein kinase) in the presence or absence of fluticasone (100 nM) and/or p38 mitogen-activated protein kinase pharmacological inhibitor SB203580."

reach
"Phosphorylation was not prevented by SB 203580 (an inhibitor of SAPK2a and p38alpha and SAPK2b and p38beta2) and/or PD 184352 (which inhibits the activation of ERK1 and ERK2), and was similar in fibroblasts lacking both SAPK3 and p38gamma and SAPK4 and p38delta or JNK1 and JNK2."

medscan
"SB203580 (10µM) treatment, which almost completely reduces Hsp27 phosphorylation by p38α/β although p38γ and p38δ are also expressed ( ), inhibited p53 mRNA (48hrs), protein expression and activity ( and ) in D-HEp3 cells."

medscan
"SB203580 concentration-dependently inhibited p38MAPK pathway and induced uPA protein expression."

medscan
"SB203580 inhibits p38α but not p38δ activity ( )."

tas
No evidence text available

medscan
"P38MAPK-blocking pharmacon SB203580 also significantly inhibited the up-regulation of BIM in CEM-C7 cells."

tas
No evidence text available

tas
No evidence text available

medscan
"SB203580 down-regulated the activity of p38MAPK pathway, but up-regulated EGR-1 expression."

sparser
"Inhibition of p38alpha by the specific inhibitor SB 203580 prevented activation-induced cell death in T cells."

medscan
"MMP-9 secretion was decreased 53.2% by inhibition of the p38 mitogen-activated protein kinase by SB203580 (P < 0.01) and 48.3% by inhibition of extracellular signal-regulated kinase with PD98059 (P < 0.05)."

tas
No evidence text available

tas
No evidence text available

tas
No evidence text available

tas
No evidence text available

tas
No evidence text available

medscan
"Bay 11-7082-induced neutrophil survival was dependent on p38-MAPK kinase activity, as the p38 kinase activity inhibitor SB203580 abrogated this response completely."

tas
No evidence text available

medscan
"Inhibition of p38 mitogen-activated protein kinase using SB203580 and small interfering RNA transfection resulted in a significant reduction in BFT-induced I kappaB kinase /NF-kappaB activation and hBD-2 expression."

medscan
"Inhibition of p38 mitogen-activated protein kinase by SB 203580 had no influence on ajoene-mediated apoptosis."

tas
No evidence text available

medscan
"Moreover, these responses are maintained in the presence of SB203580, an agent that inhibits p38 alpha and beta, further suggesting a central role for the p38 delta isoform."

reach
"SB203580 has been used to inhibit MAPK14 and p38-alpha and MAPK11 and p38-beta with IC 50 = 50 nM and 500 nM respectively XREF_BIBR, XREF_BIBR."

reach
"The compound SB203580 specifically inhibits p38 and CSBP kinase activities, whereas PD098059 selectively blocks activation of the ERK kinase MEK1, which in turn prevents activation of ERK1/2."

medscan
"We discovered that both PAR1 and P2Y1 promote p38 activation through autophosphorylation, since the process was inhibited by SB203580, which blocks p38-α and p38-β activity but does not perturb phosphorylation of p38 mediated by upstream MAPKs ( ; )."

sparser
"Moreover, the pyridinyl-imidazole compounds SB 203580 and SB 202190 inhibit SAPK2a and SAPK2b, but not SAPK3 and SAPK4 ."

tas
No evidence text available

medscan
"Next, we blocked p38 signaling with SB203580, a pyridinyl imidazole that inhibits the kinase activity of p38α and p38β without affecting p38 phosphorylation."

reach
"XREF_BIBR In addition, inhibition of MAPK14 by SB203580 has previously been associated with autophagic cell death as characterized by the accumulation of large cytosolic vacuoles."

tas
No evidence text available

sparser
"SB 203580 inhibits p38α, β and β2 (not γ and δ) MAP kinase by competing with the substrate ATP [ xref , xref ]."

tas
No evidence text available

medscan
"The specific inhibitor of p38 MAPK (SB203580), and silencing of p38α MAPK by small interfering RNAs , but not ERK1/2 inhibitor (PD98059) blocked the stimulatory effects of Berberine on protein expression of p53 and FOXO3a."

tas
No evidence text available

medscan
"Interestingly, inhibition of p38 mitogen-activated protein kinase by SB203580 severely decreased induction of COX-2 expression by IL-1beta. p38 mitogen-activated protein kinase signalling was required for Interleukin-1beta-dependent stabilization of COX-2 transcript."

reach
"The observation that overexpression of wild-type S6K in HEK293 cells increases the IC 50 10-fold from that of endogenous S6K in fibroblasts raises the possibility that Ro 31-6045 also targets the inactive form of the kinase, as reported for the inhibition of MKK1 by U0126 and PD 184352 [14] and SAPK2a by SB 203580 [27]."

tas
No evidence text available

tas
No evidence text available

tas
No evidence text available

reach
"Moreover, the pyridinyl-imidazole compounds SB 203580 and SB 202190 [41] inhibit SAPK2a and SAPK2b, but not SAPK3 and SAPK4 [24]."

tas
No evidence text available

reach
"SB203580 has been well characterized to inhibit the SAPK2a and 2b isoforms of p38 by> = 90% without appreciably (< 30%) affecting related kinases such as ERK (extracellular signal regulated kinase) or JNK (c-Jun N-terminal kinase), or 17 other widely expressed kinases."

medscan
"The levels of HO-1 and p21 induced were significantly inhibited by p38 mitogen-activated protein kinase (p38 MAPK) inhibitor (SB203580) and extracellular-regulated kinase (ERK) inhibitor (PD098059)."

medscan
"Inhibition of p38 mitogen-activated protein kinase by SB203580 prevented increases in paracellular permeability, actin rearrangement, and increases in the F-/G-actin ratio caused by IFN-γ."

medscan
"Forced inhibition of p38 mitogen-activated protein kinase activity by the specific pharmacological inhibitor SB203580 or by p38 mitogen-activated protein kinase gene knockdown using small-interfering RNA counteracted the effects of FL3, demonstrating that its chemopreventive action is related to growth inhibition and a p38-dependent caspase-3-dependent induction of apoptosis in Oct4-expressing Cancer stem cells."

tas
No evidence text available

medscan
"Unlike SAPK2a and SAPK2b, SAPK4 and SAPK3 were not inhibited by the drugs SB 203580 and SB 202190."

medscan
"Knockdown of HSP27 and PKDs markedly inhibited VEGF-induced Human Umbilical Vein Endothelial Cells migration and tubulogenesis, whereas inhibition of the p38 kinase pathway using either SB203580 or siRNAs against p38alpha or MAPKAPK2, had no significant effect on the chemotactic response to VEGF."

tas
No evidence text available

signor
"Pretreatment of hela cells with sb 203580, a pyridinyl imidazole compound that specifically inhibits p38 mitogen-activated protein kinase (mapk). It has previously been established that sb 203580 acts primarily to block the catalytic activity of p38 mapk. However, it has been suggested that in cells, the compounds could also inhibit p38 mapk activation by virtue of their ability to bind to the inactive enzyme."

tas
No evidence text available

tas
No evidence text available

tas
No evidence text available

reach
"It is not blocked by SB 203580, which inhibits SAPK2a and p38alpha and SAPK2b and p38beta but not SAPK3 and p38gamma, or by the ERK pathway inhibitor PD 184352."

medscan
"Furthermore, suppression of extracellular signal-regulated kinases and p38 mitogen-activated protein kinase activity by their specific inhibitors, U0126 and SB203580, abrogated CD24-induced proliferation in vitro."

tas
No evidence text available

tas
No evidence text available

medscan
"Inhibition of p38 mitogen-activated protein kinase by SB203580 attenuated Oxidized low density lipoprotein-induced calcification of vascular smooth muscle cells."

tas
No evidence text available

medscan
"By contrast, direct inhibition of p38 mitogen-activated protein kinase (inhibitor SB203580) efficiently suppresses IL-8 with potency comparable with dexamethasone."

medscan
"Interestingly, a specific phosphoinositide 3-kinase /Akt inhibitor (LY294002) augmented the As4O6 induced cell death; whereas p38 mitogen-activated protein kinases (p38 MAPK) inhibitor (SB203580) abrogated the cell death."

sparser
"P38α and p38β are inhibited by SB 203580 and SB 202190, with IC 50 s of 0.092 μM and 1 μM, respectively."

medscan
"Interestingly, activation of CD30 induced a strong synergistic reduction of cell activity, if the p38 mitogen-activated protein kinase activity was blocked by SB203580."

tas
No evidence text available

medscan
"Additionally, we showed that following DNA damage, activation of p38 kinase was significantly elevated, while Erk kinase activation was decreased in mutant cells, and treatment of SHP-2 mutant cells with SB203580, a selective inhibitor for p38 kinase, partially restored the DNA damage-induced G2 arrest response."

reach
"Glycogen synthase becomes phosphorylated at Ser644 in response to osmotic shock; this phosphorylation is prevented by pretreatment of the cells with SB 203580, which inhibits SAPK2a and p38a and SAPK2b and p38b activity."

medscan
"ERK1/2 phosphorylation was blocked by U0126 but not by SB203580, and p38(MAPK) activity was reduced by SB203580 but not by U0126."

tas
No evidence text available

medscan
"The involvement of p38 mitogen activated-protein kinase in the up-regulation of IL-6 and IL-8 by HIV-1 Tat was determined by pretreating the astrocytes with SB203580, a specific inhibitor of p38 mitogen activated-protein kinase. p38 mitogen activated-protein kinase exists in four different isoforms (α/β/γ/δ), of which SB203580 inhibits the p38α and p38β isoforms [ ]."

signor
"Pretreatment of hela cells with sb 203580, a pyridinyl imidazole compound that specifically inhibits p38 mitogen-activated protein kinase (mapk). It has previously been established that sb 203580 acts primarily to block the catalytic activity of p38 mapk. However, it has been suggested that in cells, the compounds could also inhibit p38 mapk activation by virtue of their ability to bind to the inactive enzyme."

tas
No evidence text available

reach
"SB203580, a pyridinyl imidazole which inhibits SAPK2a [34], has been widely used to implicate this signalling pathway in the phosphorylation of eIF4E [2,3,5,16,18-21,28]."

medscan
"To determine if thrombin activates p38 through the canonical three-tiered kinase cascade, we used the p38 inhibitor SB203580, which specifically inhibits the catalytic activity of p38-α and -β isoforms without blocking p38 phosphorylation mediated by the upstream kinases ( )."

tas
No evidence text available

sparser
"Unlike SAPK1 (also called JNK), SAPK3 and SAPK4 did not phosphorylate the activation domain of c-Jun. Unlike SAPK2a and SAPK2b, SAPK4 and SAPK3 were not inhibited by the drugs SB 203580 and SB 202190."

trips
"Although CA treatment decreased the phosphorylation of p38 mitogen‑activated protein (MAP) kinase and p53, p38 MAP kinase inhibition by SB203580 did not affect SM‑induced cell death."

tas
No evidence text available

medscan
"The chemical inhibitor SB203580 inhibits the p38α and p38β isoforms, but not the p38γ or p38δ isoforms ."

tas
No evidence text available

tas
No evidence text available

medscan
"In contrast, cell migration and p38 MAP kinase activation, in response to Sphingosine 1-phosphate, were inhibited by SB203580 but not by PD98059."

medscan
"Inhibition of p38 mitogen-activated protein kinase (by SB202190 and SB203580) or a dominant-negative mutant of Sema-3A receptor plexin-A1 reversed the inhibitory effects of VEGF in normal mesothelial cells, increasing cyclin D1 synthesis and cell growth."

tas
No evidence text available

tas
No evidence text available

medscan
"SB203580 was used to inhibit the p38 mitogen-activated protein kinase signaling pathway."

reach
"The necrostatin-1 inhibits RIP1 kinase activity, which is required to trigger necroptosis pathway."

reach
"Moreover, necrostatin-1, an inhibitor that targets RIP-1, has been shown to exert neuroprotective functions and to shield neurons from cell death following CCI-TBI."

reach
"Furthermore, heme mediated cell death is unaffected by the addition of necrostatin-1 (a specific inhibitor of RIP1) (results not shown)."

reach
"Necrostatin-1 (an inhibitor of RIPK1, RIPK3-KO, and MLKL-KO) mice were recently used to demonstrate the role of necroptosis in IR- and cisplatin induced AKI."

reach
"The RIP1 antagonist necrostatin-1 prevented the loss of ES-MNs exposed to either mutSOD1 or sALS (XREF_FIG) astrocytes."

sparser
"Necrostatin-1 inhibits RIPK1 by blocking its association with RIP3."

reach
"Necrostatin-1 blocks both RIPK1 and IDO : consequences for the study of cell death in experimental disease models."

reach
"Inhibition of RIP1 by Necrostatin-1 partially rescued cells from imatinib deprivation induced cell death, indicating that this necrotic and necroptotic signaling pathway substitutes for the blocked apoptosis at least partially."

sparser
"In contrast, RIP1 expression in mouse liver remained unchanged following ethanol feeding and inhibition of RIP1 kinase by necrostatin-1 did not attenuate ethanol-induced hepatocyte injury."

reach
"We also included necrostatin-1, which inhibits RIP1 kinase activity and therefore necroptosis."

reach
"The LPS or bacterial induced necroptosis in wild type BMDMs was also inhibited by Necrostatin-1 (Nec-1), indicating a RIPK1 kinase dependency (XREF_SUPPLEMENTARY)."

reach
"Inhibition of RIP1 by necrostatin-1 did not inhibit A1-IRS activity, thereby excluding necroptosis (XREF_SUPPLEMENTARY)."

reach
"Necrostatin-1 inhibits the kinase activity of RIP1 26 while necrosulfonamide targets MLKL, which has recently emerged as a key signaling protein that mediates binding to downstream targets."

reach
"Inhibition of RIP1 by necrostatin-1 clearly protected from TNF induced necroptosis in all three L929 sublines, but had no effect on the course of MNNG induced necrosis over 16h."

sparser
"Necrostatin-1 specifically inhibits RIP1 kinase and disrupts regulated necrosis in tissue culture xref – xref ."

reach
"ZVAD-FMK (an inhibitor of pan-caspase), necrostatin-1 (a selective inhibitor of RIPK1), and chloroquine (an inhibitor of autophagy by raising the lysosomal pH) can not block ferroptosis [XREF_BIBR]."

reach
"Additionally, while the effect of Y100 was abrogated by necrostatin-1 (an inhibitor of the necroptosis effector RIPK1), neither siRNA mediated knockdown of RIPK1 nor inhibition of RIPK3 with GSK ' 872 was sufficient to prevent Y100 mediated cell death (XREF_SUPPLEMENTARY and XREF_SUPPLEMENTARY)."

reach
"Recently, receptor interacting protein-1 (RIP1)-dependent necrosis, which is inhibited by necrostatin-1, an inhibitor of RIP1, has emerged as a form of regulated necrosis."

reach
"XREF_BIBR and XREF_BIBR Although inhibition of necroptosis by RIP3 deficiency, RIP1 blockade by necrostatin-1, or MLKL deficiency all reduce cell death at early time points, XREF_BIBR and XREF_BIBR RIP3 deficiency was unable to abrogate acetaminophen induced liver injury at 24 hours."

reach
"In addition, necroptosis can be manipulated by inhibitors such as Necrostatin 1, which blocks RIP1 kinase activity."

reach
"Furthermore, we tested an inactive analog of necrostatin-1, necrostatin-1I, which does not inhibit RIPK1 or necroptosis in other settings."

reach
"Necrostatin-1 administration inhibited the upregulation of RIP1 and RIP3 and enhanced the expression of caspase-8 in DSS induced colitis."

sparser
"Inhibition of RIP1 by necrostatin-1 did not inhibit A1-IRS activity, thereby excluding necroptosis ( xref ). xref We also looked for mitochondrial perturbation and did not observe any cytochrome C redistribution ( xref ) or ROS increase ( xref ) after incubation with A1-IRS, and mitochondria appeared normal when observed under electronic microscopy (data not shown)."

reach
"9 Generation of the kinase receptor interacting protein 1 (RIP1)/RIP3 containing ' necrosomes ' activates downstream necroptotic signaling, which can be inhibited by necrostatin 1 (XREF_FIG)."

reach
"Inhibition of RIP-1 by Necrostatin-1 also did not alter cell death in male or female cells (not shown)."

sparser
"Indeed, inhibition of RIP1 by necrostatin-1 abolishes both apoptosis and necrosis in RPE cells."

sparser
"Here, we investigated whether inhibiting RIPK1 by necrostatin-1 (Nec-1) could limit neuroinflammation and attenuate POCD in D-Galactose (D-Gal)-induced aged mice."

sparser
"Necrostatin-1 (Nec-1) inhibits receptor-interacting protein 1 (RIP1) kinase and programmed necrosis."

sparser
"We found that RIP-1 expression was upregulated in the lung of ALI mice and inhibition of RIP-1 by necrostatin-1 significantly prolonged the survival time of mice, which was accompanied by less serve lung injury."

sparser
"Generally, necroptosis is defined as cell death mediated through a pathway that depends on the RIPK1-RIPK3 complex that may be inhibited by necrostatin-1 (Nec-1) ( xref )."

reach
"In an OS model, RIPK1 mediated necroptosis was confirmed as the main cell death mechanism involved in Shikonin therapy, as only Necrostatin-1 (an inhibitor of RIPK1) was able to induce treatment reversion."

reach
"6 Necrostatin-1 (Nec-1) prevents necroptosis by binding to and inactivating RIP1."

sparser
"Inhibition of RIP-1 by Necrostatin-1 also did not alter cell death in male or female cells (not shown)."

reach
"Since necrostatin-1 has been shown to block not only RIPK1 but also indoleamine 2,3-dioxygenase (IDO), the IDO2 specific inhibitor 1-methyl-D-tryptophan (1-MT) was added to BMDC cultures as a control."

reach
"In line with its essential role in programmed necrosis, pharmacological inhibition of RIPK1 by necrostatin-1 protected the same subset of sensitive tumor cell lines that we had used for analysis in Figure XREF_FIG d (resistant KNS-62 cells were again included as control) from both TRAIL/zVAD/CHX- and TNF/zVAD/CHX induced programmed necrosis."

sparser
"Inhibition of RIPK1 by Necrostatin-1s (Nec1s) resulted in reduced, but not totally inhibited, caspase activation and cell death, demonstrating simultaneous occurrence of RIPK1 kinase-dependent and -independent apoptosis (Fig. xref )."

reach
"64 Indeed, genetic deletion of RIPK3 in Casp8 -/- dendritic cells rescues LPS induced IL-1beta production from Casp8 -/- dendritic cells, and necrostatin-1 (a chemical inhibitor of RIPK1 kinase activity) treatment inhibits LPS induced IL-1beta production from Casp8 -/- dendritic cells."

sparser
"Necroptosis was significantly reduced when RIP1 kinase was inhibited by necrostatin-1, whereas inhibition of caspases with z-vad-fmk, an irreversible pan-caspase inhibitor, did not prevent necroptosis."

reach
"Necroptosis induction requires the activity of RIP-1 kinase, which can be repressed by necrostatin-1 (Nec-1)."

reach
"Indeed, inhibition of RIP1 by necrostatin-1 abolishes both apoptosis and necrosis in RPE cells."

sparser
"Inhibition of RIP1 by necrostatin-1 clearly protected from TNF-induced necroptosis in all three L929 sublines, but had no effect on the course of MNNG-induced necrosis over 16 h (Fig.  xref b)."

tas
No evidence text available

reach
"Though Necrostatin-1 has been widely used to inhibit RIPK1 kinase activity, off-target effects have been reported."

reach
"The pharmacologic small molecule necrostatin 1 inhibits RIPK1 and prevents necroptosis in most cellular models -- a very useful experimental tool."

reach
"26 Here, we show that LTX-315 also can induce ICD in the context of a necrotic pathway that is independent of RIP3 (and is not inhibited by necrostatin 1, which inhibits RIP1), XREF_BIBR, XREF_BIBR suggesting that different pathways leading to necrosis can cause ICD."

tas
No evidence text available

sparser
"Moreover, RIP1 inhibition by necrostatin-1 only reduces mitochondrial but not cytosolic ROS elevation (Fig.  xref )."

reach
"Necrostatin-1 (Nec-1) specifically blocks the kinase activity of RIPK1 [XREF_BIBR]."

reach
"Necrostatin-1 specifically inhibits RIP1 kinase and disrupts regulated necrosis in tissue culture XREF_BIBR - XREF_BIBR."

reach
"This is consistent with the notion that Necrostatin-1 inhibits RIPK1 kinase activity, which is required for the formation of RIPK1/FADD/caspase -8 complex (Degterev et al., 2008; Wang et al., 2008)."

reach
"This TNF-alpha and Fas-induced cell death is a type of receptor interacting protein-1 (RIP-1)-dependent programmed necrosis called necroptosis, which can be prevented by necrostatin-1, a specific RIP-1 inhibitor."

sparser
"Inhibition of RIP1 by Necrostatin-1 partially rescued cells from imatinib deprivation-induced cell death, indicating that this necrotic/necroptotic signaling pathway substitutes for the blocked apoptosis at least partially."

reach
"20 Necrostatin-1 and an optimized derivative of necrostatin-1 (7-Cl-O-Nec-1) are small molecules inhibitors of RIP1."

sparser
"Inhibition of RIP1 by Necrostatin-1 and even more effectively p38-MAPK inhibition by the p38 inhibitor III rescued cells from imatinib deprivation induced cell death ( xref , right panel), indicating that these proteins play a major role for cell death upon oncogenic stress."

reach
"Z-VAD-FMK (a caspase inhibitor) and necrostatin-1 (a specific inhibitor of RIPK1) were utilized to determine whether the cell death was mediated by RIPK1 or caspases."

reach
"In the second approach, we turned to a fragment based strategy, combining a hinge binding fragment of ponatinib with a highly selective necrostatin-1 inhibitor of RIPK1."

reach
"Necrostatin-1 blocks necroptosis in many settings by interacting and inhibiting RIPK1 kinase activity, which is required for activation of RIPK3."

sparser
"Inhibition of RIP-1 by Necrostatin-1 (Nec-1) in SW620 cells ameliorated cell death and AIF translocation after AA005 treatment (Figure  xref B, C), whereas AIF knockdown failed to affect the increase in RIP-1 evoked by AA005 (Figure  xref D)."

reach
"And, necrostatin-1 could effectively reverse the changes of pulmonary fibrosis, RIPK1, RIPK3, and ECM in vivo and in vitro experiments."

reach
"To determine if necroptosis was involved in the neurotoxicity induced by miR-21, we treated the cultures with necrostatin-1, which specifically inhibits RIPK-1."

reach
"In vitro, human neutrophils fed CA-MRSA lyse by an unknown mechanism that is inhibited by necrostatin-1, an allosteric inhibitor of receptor interacting serine/threonine kinase 1 (RIPK-1)."

sparser
"Inhibiting RIPK1 and RIPK3 by their inhibitors Necrostatin-1 (Nec-1) and GSK-872, respectively, showed effective activities against osteoclast differentiation and bone resorption induced by receptor activator of nuclear factor-κB ligand (Rankl)."

reach
"Inhibition of RIP-1 by Necrostatin-1 (Nec-1) in SW620 cells ameliorated cell death and AIF translocation after AA005 treatment, whereas AIF knockdown failed to affect the increase in RIP-1 evoked by AA005."

sparser
"Thus, we here induced aged mice via intraperitoneal injection of D-Gal for 2 months and detected the effects of inhibiting RIPK1 by necrostatin-1 (Nec-1; Yang et al., xref ) on POCD in the aged mice."

reach
"In particular, necrostatin-1 has been identified to specifically inhibit the kinase activity of RIP1, thereby undermining its interaction with RIP3 and antagonizing necroptosis, without affecting NF-kappaB XREF_BIBR."

reach
"In a previous study, we showed that expression of the tnt gene in Jurkat T cells (Jurkat 655-TNT) induced a necrotic like cell death that was not prevented by necrostatin-1, indicating RIPK1 independent necrosis."

reach
"S. aureus can also trigger necroptosis, cell death dependent on RIPK1, RIPK3, and MLKL that can be blocked by necrostatin-1 and necrosulfonamide (NSA)."

reach
"We found that RIP-1 expression was upregulated in the lung of ALI mice and inhibition of RIP-1 by necrostatin-1 significantly prolonged the survival time of mice, which was accompanied by less serve lung injury."

reach
"Furthermore, necrostatin-1 significantly downregulated necroptosis related proteins (MLKL, RIP1, and RIP3) and apoptosis related proteins (cleaved-Caspase-3, Bax), and it upregulated the expression of anti-apoptotic protein Bcl-2."

reach
"A necrostatin-1 dose response for the concentration of necrostatin-1 required to inhibit the RIP1 activity in U1 cells was determined by EGFRvIII driven phosphorylation of phospho-p65 (XREF_SUPPLEMENTARY)."

reach
"Necroptosis was significantly reduced when RIP1 kinase was inhibited by necrostatin-1, whereas inhibition of caspases with z-vad-fmk, an irreversible pan-caspase inhibitor, did not prevent necroptosis."

reach
"However, it is certain that Ser161 contributes to necrostatin-1 (Nec-1)-mediated inhibition of RIP1 kinase activity."

reach
"DAPE induced reduction of NCI-H28 cell viability was partially inhibited by necrostatin-1, an inhibitor of RIP1 kinase to induce necroptosis, or knocking-down RIP1."

reach
"Conversely, necroptosis is dependent on RIP1 kinase activity, which can by inhibited by necrostatin-1 (nec-1)."

reach
"Necrostatin-1 (Nec-1) inhibits receptor interacting protein 1 (RIP1) kinase and programmed necrosis."

sparser
"In line with its essential role in programmed necrosis, pharmacological inhibition of RIPK1 by necrostatin-1 protected the same subset of sensitive tumor cell lines that we had used for analysis in Figure  xref d (resistant KNS-62 cells were again included as control) from both TRAIL/zVAD/CHX- and TNF/zVAD/CHX-induced programmed necrosis (Figure  xref a)."

reach
"By using a chemical approach, we showed that pretreatment of mice with Necrostatin-1 (Nec-1) (inhibitor of RIPK1) and/or PJ34 (inhibitor of PARP-1) significantly protected mice against concanavalin A (ConA) liver injury (aspartate amino-transferase (AST)/alanine amino-transferase (ALT)) associated with down-regulated hepatocyte specific IL-33 expression."

reach
"Necrostatin-1 specifically inhibits the pro necrotic RIP1 kinase activity, but not the kinase activities of other kinases including RIP2 and RIP3 [XREF_BIBR, XREF_BIBR]."

reach
"Necrostatin-1 treatment abrogated association of RIPK1 and MLKL with ATG5 (XREF_FIG), indicating a requirement for RIPK1 kinase activity for complex formation."

sparser
"However, inhibiting Ripk1 directly by necrostatin-1 did not rescue cell death induced by N-Gsdma3, indicating that mitochondrial targeting is crucial for Gsdma3 activation."

reach
"In addition, it was identified that the increased RIPK1 and RIPK3 can be inhibited by necrostatin-1 in both in vivo and in vitro."

reach
"Inhibition of RIP1 by Necrostatin-1 and even more effectively p38-MAPK inhibition by the p38 inhibitor III rescued cells from imatinib deprivation induced cell death (XREF_FIG, right panel), indicating that these proteins play a major role for cell death upon oncogenic stress."

reach
"Moreover, RIP1 inhibition by necrostatin-1 only reduces mitochondrial but not cytosolic ROS elevation."

sparser
"In addition, it was identified that the increased RIPK1 and RIPK3 can be inhibited by necrostatin-1 in both in vivo and in vitro ."

sparser
"Inhibiting RIPK1 by necrostatin-1 strikingly mitigated cognitive impairment and alleviated postoperative amplified neuroinflammation, necroptosis and GluA1 loss in hippocampus."

sparser
"However, it should be noted that, although inhibition of RIP1 kinase by necrostatin-1 did not have an effect on the cytotoxic activity of NaD1, the ability of NaD1 to effect the necroptotic pathway downstream of RIP1 kinase activity, such as via direct modulation of MLKL-mediated membrane disruption, xref not addressed in our current study should be determined to eliminate definitively necroptosis as having a role."

reach
"Necroptosis is another pathway leading to programmed cell death; necroptosis is inhibited by necrostatin-1 which blocks RIP1 kinase, an essential component of the pathway [XREF_BIBR, XREF_BIBR]."

reach
"Mechanistically, we show that inactivation of RIP1 by necrostatin-1 suppressed nuclear factor kappaB (NF-kappaB) cascade signals, including activation of IKKalpha, nuclear factor kappa B inhibitor protein alpha (IkappaBalpha), accumulation of nuclear p65 and NF-kappaB promoter activity."

reach
"Further, Necrostatin-1 (Nec-1) -- an inhibitor of RIP1 kinase activity -- dramatically increased baculoviral transgene expression in RIP1 silenced cells."

signor
"The interaction between rip1 and rip3 is abolished by the rip1 kinase inhibitor necrostatin-1."

reach
"In rodents, it has previously been shown that necrostatin-1 (Nec-1) inhibits RIP1, a central regulator of programmed necrosis, thereby decreasing cell death and reducing infarct size (IS) after ischaemia and reperfusion (I/R) injury."

reach
"Necrostatin-1 blocks the association of RIP1 with RIP3, leading to RIP3 instability, and cleavage from a 57-kDa to a 55-kDa protein."
2 | 89

reach
"In addition, MSL significantly inhibited nuclear translocation of NF-kappaB p65 in cells treated with LPS in vitro."

reach
"Our results showed that LPS treatment caused the decrease of p65 and p50 in the cytoplasm and increase in nucleus, and this effect can be reversed by the pretreatment with WEL in a dose dependent manner."

reach
"As shown in XREF_FIG, LPS induced p65 nuclear translocation was significantly blocked in GRK5 -/- cells compared to GRK5 +/+ macrophages (XREF_FIG)."

reach
"LPS induced secretion of TNF-alpha, IL-6, IL-1beta and NO was significantly attenuated in RelA and p65 Deltamye BMMPhis compared to WT (XREF_FIG)."

reach
"Our results indicate a reduction of approximately 50% in the amounts of nuclear p65 in DC treated with DHA and LPS, and the reduction in p65 nuclear translocation correlates with DHA mediated IkappaB stabilization."

reach
"Taken together, our results suggested that sauchinone could inhibit production of NO in LPS stimulated RAW264.7 cells through the suppression of NF-kappaB by inhibiting transactivation activity of RelA subunit."

reach
"GILZ-p Inhibits LPS Induced NF-kappaB p65 Nuclear Translocation."

reach
"XREF_BIBR, who demonstrated that Ni 2+ ions inhibited LPS induced p65 binding to DNA in a human monocyte cell line."

reach
"The LPS induced p65 translocation was inhibited by EP treatment."

reach
"Other flavonoids, quercetin and cyanidin, which lack alpha,beta-unsaturated carbonyl group, showed weaker or no inhibitory effect on both IL-6-induced STAT3 phosphorylation and LPS induced p65 translocation."

reach
"When p100 expression is high due to BCR stimulated RelA and cRel activity, the latter mechanism might lead to post-induction repression of cRel akin to IkappaBdelta mediated repression of lipopolysaccharide induced RelA activity in mouse embryonic fibroblasts (MEFs)."

reach
"Dipyrone and 2,5-dimethylcelecoxib suppressed LPS induced p65 and JNK MAPK (C-Jun N-terminal kinase mitogen activated protein kinase)."

reach
"In addition, LPS induced p65 nuclear translocation was significantly suppressed by NOX4 siRNA."

reach
"Taken together, these results demonstrate that the induction of HO-1 has an inhibitory effect on LPS induced COX-2 expression through the attenuation of LPS induced p65 translocation."

reach
"This treatment pronouncedly alleviated LPS induced neuroinflammatory response, characterized by decreased 5LO, suppressed activation of microglia, decreased NF-kappaB p65, TNF-alpha and IL-1beta, and significantly increased the ratio of p-CREB and CREB or mBDNF and proBDNF in the hippocampus of the LPS challenged mice."

reach
"Finally, we report that news SERMs, ospemifene and bazedoxifene, exert anti-inflammatory actions by a mechanism involving classical estrogen receptors and by the inhibition of LPS induced NFkappaB p65 transactivation."

reach
"Moreover, the enhancement of stat3 phosphorylation and decrease of LPS induced p65 translocation were achieved by nicotine treatment."

reach
"We conclude that LPS tolerance disrupts the transactivating stage of NF-kappaB p65 and altered nucleosome remodeling at the IL-1beta promoter in human leukocytes."

reach
"LPS also reduced cytoplasmic NF-kappaB p65 while elevated nuclear NF-kappaB p65 (P < 0.01, Figure XREF_FIG, XREF_FIG), indicating that LPS stimulated nuclear translocation of p65 and activated NF-kappaB signaling."

reach
"Inhibition of LPS induced NF-kappaB p65 nuclear translocation was confirmed by immunoblotting (data not shown)."

reach
"In fact, IkappaB kinase (IKK) assays showed that 1,25 (OH) 2 D 3 attenuated LPS induced IkappaBalpha phosphorylation in macrophages (XREF_FIG), which explains why 1,25 (OH) 2 D 3 stabilized IkappaBalpha protein reported previously, and immunostaining confirmed that 1,25 (OH) 2 D 3 blocked LPS induced p65 nuclear translocation RAW264.7 cells (XREF_FIG)."

reach
"We found that propofol treatment significantly (p < 0.05) reduced LPS induced NF-kappaB p65 nuclear translocation (47.3 +/-10.2 with LPS only vs. 17.1 +/-4.5 with LPS + propofol, XREF_FIG)."

reach
"By performing ChIP-qPCR on the selected IL-4-reduced p65 binding associated enhancers, we confirmed that IL-4-dependent reduction of LPS induced p65 binding was completely abolished in the absence of STAT6 (XREF_SUPPLEMENTARY B)."

reach
"Moreover, we elucidated that MBF-DE attenuated LPS induced inflammatory responses by blocking activation of both NF-kappaB and p65 and pERK and MAPK pathways."

reach
"PTX similarly decreased LPS induced NF-kappaB p65 nuclear phosphorylation, a marker of NF-kappaB activation and nuclear translocation (100 +/- 0 vs. 40 +/- 6; P = 0.03; XREF_FIG)."

reach
"LPS stimulated NF-kappaB p65 subunit nuclear translocation, visualized by immunofluorescence, was attenuated in both MS and T2DM subjects after MES+HS treatment (XREF_FIG C and XREF_FIG C)."

reach
"In our study, LPS induced degradation of IkappaB-alpha and NF-kappaBp65 nuclear localization in BMDCs from Wnt5aCAGDelta and Delta and Ror2 MxDelta and Delta mice, as well as BMDCs from control mice."

reach
"Our data revealed that LPS induced the up-regulation of ICAM-1, MCP-1 and TNF-alpha, LPS induced the down-regulation of PPARgamma, and LPS enhanced NF-kappaB p65 activation and DNA binding activity were substantially suppressed by emdoin in RAW264.7 cells."

reach
"However,