"Leupeptin and p-chloromercuribenzoate inhibited both cathepsin B and collagenolytic cathepsin in the ciliary body lysosomes."
"However, interpretation of the results obtained on whole tissue has been complicated by the fact that antipain and leupeptin are potent inhibitors of lyso- somal cathepsin B, as well as the sarcoplasmic CDP (Umezawa, 1976)."
"During the time that cathepsin B plus L activities in autophagic vacuoles are inhibited by the injection of leupeptin, cytosolic enzymes are being accumulated in autophagic vacuoles suggesting that leupeptin blocks intralysosomal proteolysis, and that cytosolic enzymes are sequestered continuously into autophagosomes."
"Because cathepsins are the principal lysosomal proteases, we used cathepsin specific inhibitors, such as leupeptin, E-64 and pepstatin, to determine the relative importance of different cathepsins in degrading MT. The study reveals that cathepsin B and/or L is (are) probably the most important enzyme (s) in degrading hepatic MT, because leupeptin, which blocks cathepsin B and L activity, inhibited the degradation of apo-MT by about 80%."
"Barrett [35] has shown that leupeptin inhibits human cathepsin B1."
"Livers of non starved rats were perfused cyclically for 2 h in the presence of either 4 x the normal concentration of amino acids (known to suppress proteolysis to basal level), or the same medium together with leupeptin (an inhibitor of cathepsin B, H and L)."
"Leupeptin has broad protease specificity and may also inhibit cathepsin B; thus we tested the effect that Sm-p80 might have in CD23 cleavage."
"The thiol proteinases, cathepsin B, H and L, which are known to be inhibited by leupeptin, are apparently involved in initiating digestion of rat 125I-asialoorosomucoid within liver lysosomes."
"Both the cathepsin B activities were completely inhibited by iodoacetamide and leupeptin, but phenylmethanesulfonyl fluoride and o-phenanthroline were ineffective."
"Leupeptin and antipain inhibit some other proteases, and thus probably also cathepsin B1, in a competitive way; under our incubation conditions their inhibition was, however, practically complete."
"The extent of inactivation of cathepsin B by leupeptin and E-64 significantly decreased in the presence of SA."
"Dose dependent effects on the embryo were demonstrated using the acid bisazo dye Trypan Blue, which inhibits endocytosis, and an enzyme inhibitor of bacterial origin known as leupeptin which inhibits cathepsin B, H and L. Homogenates of rat kidney and placenta also produced congenital defects; the concommitant electron microscopical changes in the yolk sac suggest that these effects too are due to alterations in the availability or quality of histiotroph."
"Leupeptin and E-64 dose dependently inhibited both cathepsin B and cathepsin L activities, and pepstatin A strongly inhibited cathepsin D activity in rat kidney lysosomes."
"Leupeptin is a potent inhibitor of cathepsin B in vitro and is presumed to act in a similar manner in vivo."
"Leupeptin is a reversible inhibitor of cathepsin B (IC 50% 1 mumol)."
"The neutral proteinase and cathepsin B like activity were inhibited by leupeptin and not by pepstatin while the converse obtained for cathepsin D activity."
"When cathepsin D was inhibited by pepstatin, the proteolysis of 125I labeled low density lipoproteins was inhibited by more than 90%, whereas when cathepsin B was inhibited by leupeptin, the rate of proteolysis decreased by approx."
"For example, leupeptin inhibits trypsin, plasmin, thrombokinase, cathepsin B, and papain; chymostatin inhibits chymotrypsin; and elastatinal inhibits elastase."
"Injection of leupeptin or E-64 into rats actually inhibits the activities of cathepsin B and cathepsin L in the lysosomal fraction of liver and results in inhibition of turnover rate of liver aldolase (11, 20)."
"It was strongly inhibited by HgCl2, ZnSO4, iodoacetate, iodoacetamide, and N-ethylmaleimide which indicated that it is a thiol protease, and by leupeptin, a strong inhibitor of cathepsin B from other sources."
"The comparison of Ki suggested that CA074 and Z-Phe-Phe-FMK inhibited exopeptidase activity, and leupeptin inhibited the endopeptidase activity of cathepsin B more strongly."
"Both the proteinase and cytotoxic activities of the purified amoebal cathepsin B were inhibited by leupeptin and serum and activated by free sulfhydryl groups, supporting the hypothesis that both activities are characteristics of amoebal cathepsin B. Virulent strains of E. histolytica (HM-1 and Rahman) had significantly more cathepsin B activity per milligram protein than less virulent strains (HK-9, Laredo, and Huff)."
"Since homogenates of leupeptin treated muscles had decreased cathepsin B activity, this lysosomal protease may play a role in protein turnover in normal and diseased muscles."
"Administration of leupeptin abolished the increased activity of two intracellular proteases calpain and cathepsin B induced by controlled mechanical ventilation."
"Experimental assay, see https://identifiers.org/chembl.compound/CHEMBL191881"